Re: C I C L Y LY and YLD (yellow leaf disease of Areca.
Dear Dr Muralidharan,
Why are you accusing Professor Govindan of making an "early warning"
more than 10 years after he published a paper on the subject? All he
says is "symptoms almost similar to Lethal Yellowing" and that
"samples [be] tested for the presence of phytoplasma." You say
yourself, that scientists in India should not be prevented from
interacting to confirm or disprove the hypothesis - and there are
coconut pathologists who are CICLY members, both in India and
From India both Subramanya Machikoppa and Jacob Daniel have advised
him to contact CPCRI and other laboratories for electron microscopy,
Diens, DAPI or ELISA tests but I believe that the Professor is already
aware of those options.
He is also aware that identifying the presence of phytoplasma by these
methods will not be sufficient to show whether or not they are are
closely related to the LY variants. For that it will be necessary to
do the sort of tests that are routinely carried out by Nigel Harrison
in the USA, Phil Jones in UK, Michel Dollet in France and other CICLY
But I do have one criticism to put to Professor Govindan - please to
provide full references for (M.Govindan et al. 1996. Indian coconut
journal. 2-4) and (Bhaskaran et al. 2007. Indian coconut Journal:
I assume the first is Govindan, M.; Peethambaran, C. K.; Muralidharan,
P. (1996) A New Disease Complex in Coconut: Progressive Chlorosis and
Crown Rot. Indian Coconut Journal 26(11) 2-4 and I would like to know
the other so that I can update Coconut Time Line.
Finally, can anyone tell me if there is yet a digital archive of
contents lists for back issues of the Indian Coconut Journal ?
Coconut Time Line <http://cocos.arecaceae.com/
On 12/10/2007, Dr. K. Muralidharan <firstname.lastname@example.org
> This is for the kind attention of all scientists:
> If a farmer is saying that there is LYD symptoms or similar to that, one can
> But a scientist should not make such early warnings. No body in India
prevents the coconut pathologists to interact and confirm or disprove the
> Muralidharan, K.
> Sr. Scientist
> subramanya_machikoppa <email@example.com
> --- In CICLY@yahoogroups.com
, "m govindan pilicode" <mgpilicode@...>
> > From,
> > Dr.M. Govindan, Professor Dept of Microbiology&Pl.Pathology.
> > Kerala Agricultural University, College of Agriculture,
> > Padnakkad. Kasargod. India.
> > Sir,
> > Greetings. In Kerala I have seen some coconut plants showing
> symptoms almost similar to Lethal Yellowing.(M.Govindan et al. 1996.
> Indian coconut journal. 2-4) Now similar reports are appearing from
> other parts of the country also(Bhaskaran et al. 2007.Indian coconut
> Journal: 2-4). I would like to have a few samples tested for the
> presence of phytoplasma. Kindly suggest the persons whom I can
> contact for help.
> > Thanking you
> > Govindan
> hello Sir,
> My advice is-u just tell to CPCRI scients. They see the sample under
> electronic microscope and tell wheather phytoplasma is present or
> The same they did in case of areca yellow leaf disease.(I have the
> book they published on it)
> CPCRI is there,near u,in Kasaragodu.
> Subramanya Machikoppa
--- In CICLY@yahoogroups.com
, jacob daniel <jacobandjacob2002@...> wrote:
> Since electron microscopy is expensive and tedious, preliminary screening of
plant samples using phytoplasma specific stains like Diens stain and DAPI stain
is advisable and more pratical. Then the samples which tested positive after
screening can be sujected to electron microscopy or ELISA Test. Facily for
electon microscopy is avaialbe at Veterrinary college KAU, Mannuthy and CPCRI
Kayamkulam. Facily for Elisa is avaialble at Dept of pl.pathology College of
Agriculture Vellayani and in Biotech Dept of KAU. Electon microscopy and ElISA
tests can complement each other in detection of phytoplasma