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CICLY · Lethal Yellowing Disease of Coconut Palm

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PCR parameter with LYF1/LYR1 primers   Message List  
Reply | Forward | Delete Message #2153 of 2180 |
Re: C I C L Y PCR parameter with LYF1/LYR1 primers


--- khmutaqin <khmutaqin@yahoo.com> wrote:

> Hello everybody,
> I am going to run PCR for detection of LY
> phytoplasma employing
> LYF1/LYR1 (Harrison et al. 1994. Plant Pathol.
> 43:998-1008). However, I
> don't have that paper. Could somebody help me to
> inform the PCR
> parameter using those primers, please!
> Thanks in advance.
> Kikin H. Mutaqin
> Dept. of Plant Protection
> Bogor Agricultural University
> Bogor - Indonesia
>
>
Dear Kikin,

In respons to your request here the parameters you
need:

30 sec (90 sec for first cycle) denaturation step at
94oC, annealing at 53oC for 50 sec and primer
extension at 72oC for 80 sec.

Hope you will get good results.

Regards

J. Sonny Warokka
Research Institute for Coconut and Other Palmae
Manado - Indonesia





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Tue Aug 7, 2007 2:44 am

Sonny Warokka <sonnywarokka@yahoo.com>
sonnywarokka
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Message #2153 of 2180 |
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Hello everybody, I am going to run PCR for detection of LY phytoplasma employing LYF1/LYR1 (Harrison et al. 1994. Plant Pathol. 43:998-1008). However, I don't...
khmutaqin
Offline Send Email
Aug 6, 2007
10:02 am

... Dear Kikin, In respons to your request here the parameters you need: 30 sec (90 sec for first cycle) denaturation step at 94oC, annealing at 53oC for 50...
Sonny Warokka
sonnywarokka
Offline Send Email
Aug 7, 2007
8:52 am

Thank you so much Dr. Warroka for the info. Kikin H. Mutaqin ... ____________________________________________________________________________________ Boardwalk...
Kikin Hamzah Mutaqin
khmutaqin
Offline Send Email
Aug 9, 2007
5:22 am

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