Re: C I C L Y PCR parameter with LYF1/LYR1 primers
--- khmutaqin <firstname.lastname@example.org
> Hello everybody,
> I am going to run PCR for detection of LY
> phytoplasma employing
> LYF1/LYR1 (Harrison et al. 1994. Plant Pathol.
> 43:998-1008). However, I
> don't have that paper. Could somebody help me to
> inform the PCR
> parameter using those primers, please!
> Thanks in advance.
> Kikin H. Mutaqin
> Dept. of Plant Protection
> Bogor Agricultural University
> Bogor - Indonesia
In respons to your request here the parameters you
30 sec (90 sec for first cycle) denaturation step at
94oC, annealing at 53oC for 50 sec and primer
extension at 72oC for 80 sec.
Hope you will get good results.
J. Sonny Warokka
Research Institute for Coconut and Other Palmae
Manado - Indonesia
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