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hugh.harries · hugh.harries@gmail.com | Group Owner  - Edit Membership Start a Group | My Groups
CICLY · Lethal Yellowing Disease of Coconut Palm

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PCR parameter with LYF1/LYR1 primers   Message List  
Reply | Forward | Delete Message #2152 of 2180 |
Hello everybody,
I am going to run PCR for detection of LY phytoplasma employing
LYF1/LYR1 (Harrison et al. 1994. Plant Pathol. 43:998-1008). However, I
don't have that paper. Could somebody help me to inform the PCR
parameter using those primers, please!
Thanks in advance.
Kikin H. Mutaqin
Dept. of Plant Protection
Bogor Agricultural University
Bogor - Indonesia




Mon Aug 6, 2007 9:56 am

"khmutaqin" <khmutaqin@yahoo.com>
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Message #2152 of 2180 |
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Hello everybody, I am going to run PCR for detection of LY phytoplasma employing LYF1/LYR1 (Harrison et al. 1994. Plant Pathol. 43:998-1008). However, I don't...
khmutaqin
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Aug 6, 2007
10:02 am

... Dear Kikin, In respons to your request here the parameters you need: 30 sec (90 sec for first cycle) denaturation step at 94oC, annealing at 53oC for 50...
Sonny Warokka
sonnywarokka
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Aug 7, 2007
8:52 am

Thank you so much Dr. Warroka for the info. Kikin H. Mutaqin ... ____________________________________________________________________________________ Boardwalk...
Kikin Hamzah Mutaqin
khmutaqin
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Aug 9, 2007
5:22 am

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