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hugh.harries · hugh.harries@gmail.com | Group Owner  - Edit Membership Start a Group | My Groups
CICLY · Lethal Yellowing Disease of Coconut Palm

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Phytoplasma transmission through seed ???   Message List  
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Dear Hugh,


We have also been investigating Phytoplasma seed transmission in Honduras in Gliricidia sepium,  a model which is a  great deal easier to study than coconut. G. sepium is a native leguminous species to Central America and Mexico and is attacked by a phytoplasma disease known as Gliricidia little leaf. We collected seed from symptomatic trees that tested PCR positive for the phytoplasma and germinated seedlings in insect-proof cages. We then tested these seedlings by nested PCR and where able to detect phytoplasma DNA, which must have come from the seed and not from insect vectors. The seedlings where then transferred to the ground and covered in much larger insect-proof cages for a year. Some plants developed mild symptoms of Gliricidia little leaf and the died suggesting that there is a self limiting mechanism in the disease spread?  We are going to continue with these studies once we get hold of some more seeds from infected trees.


And… we have also found some positive PCR samples in tissue from germinated nuts obtained from diseased palm trees with LY symptoms, but it is too early to draw any conclusions.  But don´t worry, we are working on it…



Best regards from Honduras



Maria Mercedes Roca, PhD

Plant Protection and Biotecnology Program

Zamorano University

P.O.Box 93

Tegucigalpa, Honduras

Tel:   (504)  776 6140 ext. 2362

Fax: (504)  776 6242

From: CICLY@yahoogroups.com [mailto:CICLY@yahoogroups.com] On Behalf Of hugh.harries
Sent: Miércoles, 21 de Marzo de 2007 11:07 a.m.
To: CICLY@yahoogroups.com
Subject: C I C L Y Phytoplasma transovarial transmission


Will the following report from the EPPO Reporting Service stimulate LY

2007/030 Possible transovarial transmission of 'Candidatus Phytoplasma
prunorum' by Cacopsylla pruni

The possible transovarial transmission of two phytoplasmas,
'Candidatus Phytoplasma prunorum' (associated with European stone
fruit yellows) and 'Candidatus Phytoplasma mali' (Apple proliferation
- EPPO A2 List) by their respective psyllid vectors Cacopsylla pruni
and C. melanoneura, was investigated in Italy. Results showed that C.
pruni could transmit 'Ca. Phytoplasma prunorum' transovarially, as it
could be detected in the progeny of infected females (i.e. eggs,
nymphs and newly emerged adults). It was also showed that psyllids
which had acquired the phytoplasma transovarially could then transmit
it by feeding on a healthy plum seedling. Transovarial transmission
could not be demonstrated with 'Ca. Phytoplasma mali' in the
experimental design used. Although further studies are needed, these
first results open new perspectives on the study of the epidemiology
of European stone fruit yellows. The fact that the insect is not only
a vector but also a reservoir for the phytoplasma has implications for
disease management.

Source: Tedeschi R, Ferrato V, Rossi J, Alma A (2006) Possible
phytoplasma transovarial transmission in the psyllids Cacopsylla
melanoneura and Cacopsylla pruni. Plant Pathology 55(1), 18-24.
Additional key words: epidemiology Computer codes: PHYPMA, PHYPPR

Wed Mar 21, 2007 9:10 pm

"Maria Mercedes Roca" <mmroca@zamorano.edu>
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Dear Hugh, We have also been investigating Phytoplasma seed transmission in Honduras in Gliricidia sepium, a model which is a great deal easier to study than...
Maria Mercedes Roca
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Mar 22, 2007
7:22 am

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