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hugh.harries · hugh.harries@gmail.com | Group Owner  - Edit Membership Start a Group | My Groups
CICLY · Lethal Yellowing Disease of Coconut Palm

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Early diagnosis of LY   Message List  
Reply | Forward | Delete Message #2083 of 2180 |
According to Simon Eden-Green "Recent progress in immunological
methods for the detection of phytoplasmas is likely to lead to
practical techniques for the diagnosis of coconut palms in early,
probably pre-symptomatic, stages of disease. Subject to suitably low
production costs, this could be used to improve the efficiency of
large-scale LYD surveillance and control operations. This is based on
the ability to clone a dominant phytoplasma membrane protein gene,
secA, into a bacterial expression system and to produce anti-secA
antibodies that can be used in a simple enzyme-linked immunological
assay such as a membrane-based lateral flow device. A research group
led by Dr Matt Dickinson at the University of Nottingham, UK, working
with researchers from the UK Central Science Laboratory, from Ghana,
and Dr Nigel Harrison in Florida, has amplified part of the secA gene
from coconut LYD phytoplasmas from Tanzania, Ghana and Mexico. The
resultant PCR products have been cloned, sequenced and confirmed to be
secA. These sequences are currently being cloned into an E.coli
expression vector, and the expressed protein will then be purified and
used to produce anti-secA antibodies for LYD phytoplasmas. The
antibodies will then be evaluated on samples obtained from Ghana to
confirm their ability to detect phytoplasma in vivo. It is thought
that this work could be extended and practical techniques developed
within a 12-18 month timescale for use in Mozambique and elsewhere,
subject to availability of further funding, some of which may come
from a new UK Biology and Biotechnology Science Research Council
scheme operating with the UK Department for International Development".

Source:
http://www.mcc.gov/about/reports/other/mcc-2006-clydreport.pdf




Wed Jan 10, 2007 9:54 pm

"hugh.harries" <harrieshc@yahoo.com>
hugh.harries
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According to Simon Eden-Green "Recent progress in immunological methods for the detection of phytoplasmas is likely to lead to practical techniques for the...
hugh.harries
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Jan 10, 2007
10:44 pm

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